Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli B CpXΔW (LMBP 13321)
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| Species: | Escherichia coli |
| Group: | B |
| Strain designation: | CpXΔW |
| Accession number: | LMBP 13321 |
| Depositor: | Dr T. Mathes1. 1 Experimental Biophysics, Institute of Biology, Humboldt University of Berlin, Berlin, Germany |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | c037 |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 28°C |
| Genotype: | F- ompT gal dcm hsdSB (rB- mB-) (DE3) manX::ribM trpA- |
| Phenotype: | Tryptophane auxotrophy |
| Properties: | The strain is intended for amino acid specific labelled protein production. As a C41(DE3) derivative, this strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lacUV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain is deficient in the outer membrane protease OmpT which reduces the degradation of heterologous proteins expressed in the strain. |
| Further information: | This strain was created based on E. coli B CmpX13 (LMBP 13314) via site specific chromosomal modification. |
| Original reference: | Mehlhorn et al., PLoS One. 8 (2013), e79006 [PMID: 24223875] [DOI: 10.1371/journal.pone.0079006] |
| Related reference: | Mathes et al., J. Mol. Biol. 385 (2009), 1511-1518 [PMID: 19027027] [DOI: 10.1016/j.jmb.2008.11.001] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli B CpXΔW (LMBP 13321) is available at BCCM/GeneCorner. It was deposited by Dr T. Mathes and was published in Mehlhorn et al., 2013. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.